Chemistry
6 min read
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Updated 2026-09-18

How Molarity and Solution Dilution Are Calculated: Moles, Liters & C1V1 = C2V2

A practical laboratory guide for chemists and students to prepare standard reagents and serial dilutions with mathematical precision.

By AnyCalcHub Chemical Sciences Desk
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1. Molarity & The Dilution Equation

Molarity is the number of moles of solute dissolved per liter of solution. When diluting a stock solution, the total amount of solute remains unchanged.
Mathematical Formula
Molarity (M) = Moles of Solute / Liters of Solution
Dilution: C₁ × V₁ = C₂ × V₂
Step-by-Step Worked Example
Stock Concentration (C₁)5.0 M
Target Concentration (C₂)0.5 M
Target Volume (V₂)500 mL
Step 1:Rearrange formula: V₁ = (C₂ × V₂) / C₁
Step 2:Calculate: (0.5 × 500) / 5.0 = 50 mL
Final Result: Measure 50 mL of 5.0 M stock and dilute to 500 mL total

Frequently Asked Questions

Common questions and mathematical nuances for this calculation.

What is the difference between Molarity (M) and Molality (m)?

Molarity is moles of solute per liter of total solution (which changes slightly with temperature), whereas molality is moles of solute per kilogram of pure solvent (temperature-independent).

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